BIOCENOSIS AND HISTAMINE INDICATORS IN ORAL FLUID AS BIOMARKERS OF THE EFFICIENCY OF PROBIOTICS CHOICE IN TREATMENT OF ORAL MUCOUS MEMBRANE DISEASES

Saygusheva L., Dudko E., Kuyarov A., Yevtushenko E.
Surgut State University, Russian Federation

Introduction:
The majority of diseases of oral mucous membrane proceed against the background of the violation of intestinal microbiocenosis. The maintenance of histamine as one of the mediators of inflammation and pain has a pathogenetic value in case of inflammatory processes. The use of probiotics realizing ecological mechanisms of microbiosenosis normalization defines the relevance of the chosen subject in theoretical and practical aspects.
The purpose of the present research is the assessment of intestinal biocenosis and histamine indicators in oral fluid as biomarkers of the efficiency of probiotics choice in treatment of oral mucous membrane diseases.

Methods:
Research was conducted on the basis of Stomatogy N1 in Surgut city and the laboratory "Fundamental problems of healthcare of the indigenous community and Northern migrants" in Surgut State University.
Two groups were under supervision, they included 67 people aged from 19 till 52 years (men - 30, women – 37) with the diagnosis recurrent aphthous stomatitis. After professional hygienic measures we used the traditional scheme of treatment of recurrent aphthous stomatitis in the comparative group (group 1; 32 persons). Patients of the main group (group 2; 35 people) received a probiotic mix orally and in the form of oral trays as a complex medical treatment.
All the patients had a clinical examination and the level of histamine in oral fluid was determined before and after treatment by the immunoenzymometric method (IEM). Examinees of the main group had a bacteriological examination of intestinal microflora for dysbacteriosis before the treatment and 30 days after it.
During the choice of probiotic strains for the increase of the efficiency of correction of violations of microflora strains of Lactobacillus bacteria were prescribed to the investigated group, they have a certain decarboxylase and antagonistic activity. The efficiency criterion of the conducted treatment was the reduction of the inflammatory activity, reduction of the period of the existence of aphthas, and also the maintenance of histamine in oral fluid.

Results:
As a result of the conducted research it was established that a positive effect, but with various degrees of expressiveness was observed in both groups. In group 2 morbidity reduction of aphthas was observed during the first two days. The period of the presence of aphthas was reduced by 2-4 days. After the conducted treatment in group No. 1 the maintenance of free histamine in saliva decreased in 30% of the investigated people and this reduction wasn’t reliable according to the average indicators(P>0,05). In group No. 2 decrease of histamine was observed in more than 90,0% of the examined people, which is a reliable indicator (Р<0,05).
During the dysbacteriosis analysis 82,8% of patients of group No. 2 tended to restore the quantity of lactobacilli, bifidobacteria and typical colibacillus. In case of 42,8% of the examined the quantity of opportunistic microorganisms has decreased by 1-2 levels.

Discussion:
Thus, the assessment of biocenosis and histamine indicators in oral fluid as biomarkers of the efficiency of probiotic treatment of oral mucous membrane diseases has shown a sufficient degree of informational content, and the use of probiotics with a certain decarboxylizing activity in complex treatment of recurrent aphthous stomatitis has considerably reduced the terms of aphthas presence. Morbidity reduction of aphthas due to the decrease in maintenance of free histamine in saliva in association with normalization of indicators of intestinal microflora can be explained by the combined intake of probiotic preparations, - both locally, and orally. It dictates the need of inclusion of probiotic preparations in a
complex treatment of recurrent aphthous stomatitis. A positive tendency of intestinal microflora normalization testifies to the need of a long intake of probiotic preparations at treatment of recurrent aphthous stomatitis for the improvement of organismal resistance.

Keywords: Biocenosis , Histamine, Probiotics, Stomatitis, Decarboxylase

Citation:
Saygusheva L., Dudko E., Kuyarov A., Yevtushenko E. (2016). Biocenosis and histamine indicators in oral fluid as biomarkers of the efficiency of probiotics choice in treatment of oral mucous membrane diseases. Conference Proceedings of IPC2016. Paper presented at the International Scientific Conference on Probiotics and Prebiotics, Budapest (p. 115.). IPC2016

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