STUDY OF THE EFFECT OF PROBIOTIC MICROORGANISMS AND THEIR ASSOCIATIONS ON THE CELLULAR IMMUNITY AND MORPHOLOGICAL COMPOSITION OF THE BLOOD OF RABBITS


Ratnikova I., Gavrilova N., Sadanov A., Bayakyshova K., Turlybayeva Z.
1RSOE, Kazakhstan

Introduction:
The published data testify to a huge role of normal intestinal microflora as factor of nonspecific resistance which is realized not only due to microbic antagonism, but also nonspecific activation of fagotsitarny and cytostatic activity of macrophages, stimulation of lymphoid fabric, impact on immunocompetent T - and B-cells.
Research objective - studying of the immunomodelling properties of probiotic microorganisms and their associations.

Methods:
In experients in vivo the the immunostimulating activity of 10 preparations from lactic and propionic acid bacteria is studied.
Researches were conducted on 44 rabbits weighing 3-3,2 kg., from calculation on four rabbits on a preparation. Prior to experiment blood of all animals was studied (hematological parameters are defined, T - and B-lymphocytes, tests with loading: NST-test, phagocytosis with latex). Examinees preparations set in a dose 10 ml on an animal in the morning in 20 minutes before feeding for 7 days. Blood for researches was taken on day 8 of the experiment from an ear vein in the morning on an empty stomach.

Results:
As a result of research of parameters of the immune status and a hematologic profile of blood of laboratory animals prior to experiment it is established that all indicators were in limits of physiological norms for this species of animals.
For the 8th day after reception of probiotic preparations the maintenance of the T-lymphocytes has increased in all experimental groups, and more with P. shermanii 2/10, L. plantarum 2v/A-6, plantaferminy and A-5 association with wheat bran. The quantity of the B-lymphocytes has practically not changed in all cases.
The maintenance of leukocytes has slightly increased in the groups accepting P. shermanii 2/10, L. plantarum 2v/A-6 and A-5 association with wheat bran. Increase in percent of lymphocytes is noted in the groups receiving L. acidophilus 27w, L. рlantarum 14d/A-24, L. plantarum – 22, A-5 association with wheat bran, monocytes – P. shermanii 2/10, L. acidophilus 27w, L. рlantarum 14d/A-24, A-5 association, granulocytes - P.shermanii 2/10, L. plantarum 2v/A-6, L. fermentum 127/A-4, B. longum 7w, L. plantarum – 22, plantapherminy, A-5 association. Also after giving the preparations P. shermanii 2/10, L. plantarum 2v/A-6, L. fermentum 127/A-4, plantapherminy, L. plantarum – 22 has increased fagocytarny activity of neutrophils, as in the spontaneous test (except for P. shermanii 2/10), and in the presence of the activator of a fagocytosis. In other studied indicators between control and experimental groups of a significant difference it was not observed.
The stated data confirm stimulation with the help of tested preparations of cellular factors of immunity within the upper bounds of physiological norm, more at the rabbits receiving the preparations P.shermanii 2/10, L. plantarum 2v/A-6, L. fermentum 127/A-4, B. longum 7w, L. plantarum – 22, plantaferminy, A-5 association with wheat bran.
Thus, preparations of lactic acid and propionic acid bacteria possess immunostimulating activity, increase the proliferation of T-lymphocytes, granulocytes, monocytes, activate bactericidal function of phagocytic cells (neutrophils) increasing their functional reserve.

Keywords: Probiotics, Lactic-acid bacteria, Bifidobacteria, Propionic-acid bacteria, Immunomodulatory properties, Cellular immunity

Citation:
Ratnikova I., et al. (2016). Study of the effect of probiotic microorganisms and their associations on the cellular immunity and morphological composition of the blood of rabbits. Conference Proceedings of IPC2016. Paper presented at the International Scientific Conference on Probiotics and Prebiotics, Budapest (p. 113.). IPC2016

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