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Salehi Jouzani G.
Agricultural Biotechnology Research Institute of Iran, Iran

The objective of the present study was to design native specific probiotic bacteria for poultry applications.

To do this, about 700 lactobacilli and bacilli bacteria were isolated from ileum of some Iranian native chickens and ducks by using selective media.

Finally, 6 Lactobacillus and 4 Bacillus subtilis strains with high probiotic potentials were selected and used in the farm experiments. The farm studies was performed to evaluate effects of the selected Lactobacillus and Bacillus strains on the yield (weight, feed conversion ratio (FCR), breast and thigh yield and feed consumption ratio) and immunological parameters (antibody titers against bronchitis and Salmonella, cholesterol and triglycerides contents, and also mucin gene (muc2) expression rate) of broilers (Ross strain) challenged or non challenged with Salmonella. The research was performed during 42 days at the framework of Factorial statistical design (5 ( Six Lactobacillus strains (106 cfu/g feed), Six B. subtilis strains (106 cfu/g feed), Lactobacillus and B. subtilis strains (106 cfu/g feed), Commercial probiotic, and Control (feed without probiotics)) x 2 (challenged or non challenged with Salmonella) by using 300 one-day old roosters fed by a defined feed regime containing one of the above mentioned probiotics. The maximum increase in the broilers yield and immunological indexes was observed for those chickens fed by feed supplements containing the Lactobacillus strains. The application of the selected Lactobacillus strains could significantly reduce the feed consumption rate (FCR) and cholesterol and triglycerides contents, and significantly increased the total broiler, breast and thigh weight in both challenged and non challenged broilers. This also significantly increased the antibody titers against bronchitis and Salmonella. The real time PCR results, also showed that the presence of Lactobacillus strains significantly increased the mucin2 (muc2) gene expression rate in the broilers in comparison to the control and other treatments.

So, the presence of probiotics (Lactobacillus, B. subtilis or commercial probiotic) could significantly increase yield and immunological parameters in the living challenged broilers. These results confirmed that the selected lactobacilli strains could be effectively used as specific probiotic bacteria for broilers at commercial level.

Keywords: Broiler chicken, Feed consumption rate (FCR), Immunological parameters, Probiotic, Mucin gene (muc2), Salmonella challenge

Salehi Jouzani G. (2016). Designing specific and efficient probiotics for broiler chickens based on native Lactobacillus strains. Conference Proceedings of IPC2016. Paper presented at the International Scientific Conference on Probiotics and Prebiotics, Budapest (p. 66.). IPC2016

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