Gebler I., Płaza G., Powałowski S., Chojniak J.
Institute for Ecology of Industrial Areas, Katowice, Poland

Nowadays more and more attention is paid to natural products, ecological lifestyle and healthy food. Probiotics play an increasingly important role in healthy eating, body care and now in agriculture. Together with growing public awareness one can observe an increasing demand for biologically active products containing beneficial microorganisms. The present study focuses on describing the process of preparing a product containing live microorganisms.

In this experiment there have been used three strains of microorganisms (E41, E42, R85), isolated, examined and identified in the project called acronym: Phyto2Energy (call identifier: FP7-PEOPLE-2013-IAPP). Having selected and chosen proper microorganisms, the next step of preparing a innoculum was adjusting appropriate substrate that would enable the growth of all the microorganisms used and ensure their stability. The experiment used waste from the agri-food industry such as: sugar cane molasses, sugar beet molasses, apple extract and original combinations of microbiological medium. The choice of substrates was dictated by the efficient growth of the microorganisms as well as economic aspects.As an additional component, 1-2% inulin was used as the form of a prebiotic. High-quality microbiological product preparation required conducting a number of bioreactor cultures controlling the following parameters: temperature, stirring speed, aeration, pressure and liquid volume. In order to assess the quality of the preparations, the bacteria growth was evaluated by the following methods: measurements of optical density, cytometry analysis and classical plating cultures.During cytometry analysis cells count and their liveliness using BacLight™ RedoxSensor™ Green Vitality Kit and propidium iodide was determined.

The conducted analysis showed that the presence of inulin in bacterial cultures as a prebiotic affected the quality and stability of the produced inoculum positively in all cases. The use of agri-food industry waste can only be applied in this case after the sterilization process because indigenous microflora tends to displace introduced microorganisms. The research showed that sugar cane molasses can be successfully used to prepare inoculum.

It is certain that the use of dietary products containing pro- and prebiotics has a positive effect on human health. However, the surrounding environment is essential for life and health of living organisms. That is why the use of beneficial microbes in agriculture and environmental protection can have a huge impact on improving the health of humans and animals. Therefore, the use of waste substrates, for instance from agri-food industry, to produce microbiological preparations dedicated to agriculture while ensuring their high quality and stability appears to be a huge chance for balanced economic development having a direct impact on human health.

Keywords: Quality Assurance and Stability, Inoculum, Prebiotic, Probiotic, Bioreactor cultures, Cytometer analysis

Gebler I., Płaza G., Powałowski S., Chojniak J. (2016). The process of preparing microbiological medium containing beneficial microorganisms ensuring product quality and stability. Conference Proceedings of IPC2016. Paper presented at the International Scientific Conference on Probiotics and Prebiotics, Budapest (p. 95.). IPC2016

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